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Light microscopy

 

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LEICA DMI 5000 microscope

The LEICA DMI 5000 microscope is an X, Y, Z automated inverted light microscope that allows you to work in brightfield, darkfield, interferential contrast in a simple way and polarized light.

Two software packages are available to process the images :

  • Multifocus that allows reconstruction in Z
  • Multistep that allows to do mapping.


Their use is illustrated by the snapshots below. The material used is a gray iron slats from water pipes in the city of Paris (Nital attack 4%)

The MultiFocus photo is obtained by overpotting 22 pictures taken in successive positions along the z axis. It makes it possible to obtain a clear image on the whole field of observation, the defects of focus due to the relief of the surface to be analyzed thus being corrected.

3 étapes intermédiaires sont pésentées ci-dessous  suivies du résultat des supperpositions des 22 photos

 

 

 

Multistep cartography : 6 images assembled

 

 

Contact : Fabrice.gaslain@mines-paristech.fr

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Zeiss Axiovert 450M reversed Light Microscope

 

The Zeiss Axiovert 405M microscope is a reversed light microscope that is able to work in bright field, dark field, polarised light and with interferential contrast. This microscope is  fitted with a 12 MPix Jenoptik Progress CF Scan numerical camera for capturing photos.

 


Bright field

Black field

Polarised light

 

 

Light microscopy - MINES ParisTech
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